An enzymic method for the assay of pyridine nucleotides in extracts of animal tissues
- 31 March 1962
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 83 (1), 191-194
- https://doi.org/10.1042/bj0830191
Abstract
The concentrations of oxidized and reduced pyridine nucleotides in tissue extracts can be estimated by an enzyme assay which couples the specific dehydrogenases and a non-specific diaphorase and measures the rate of reduction of 2,6-dichlorphenol-indophenol. The results of these assays agree well with those obtained by a fluorimetric assay and with the assays of Glock and McLean (1955) who used cytochrome c reductases and cytochrome c.Keywords
This publication has 3 references indexed in Scilit:
- Specific methods for the microdetermination of pyridine nucleotides and pyridine nucleotide enzymesArchives of Biochemistry and Biophysics, 1957
- The determination of oxidized and reduced diphosphopyridine nucleotide and triphosphopyridine nucleotide in animal tissuesBiochemical Journal, 1955
- STUDIES ON DIPHOSPHOPYRIDINE NUCLEOTIDE-CYTOCHROME-C REDUCTASE .2. PURIFICATION AND PROPERTIES1952