THE EFFECT OF CHEMICAL SYMPATHECTOMY ON MITOCHONDRIAL FUNCTION IN THE ISCHAEMIC AND REPERFUSED MYOCARDIUM

Abstract

1 Isolated rabbit hearts were perfused aerobically for 120 min, made ischaemic for 90 min, or made ischaemic for 90 min and then reperfused for 30 min. 2 Some rabbits were pretreated with 6-hydroxydopamine (6-OHDA), given as three separate intravenous doses of 30, 20 and 20 mg/kg, 20 to 48 h before they were killed; others (controls) received saline according to the same regime. 3 Mitochondria were harvested from left ventricular homogenates and their function assessed by measuring state 3 O₂ consumption (state 3 QO₂), respiratory control index (RCI), phosphate: oxygen ratio (ADP:O), Ca²⁺ content, and ATP-producing activity. In other experiments peak left ventricular developed tension was recorded. 4 In hearts from saline-treated animals, mitochondrial state 3 QO₂, RCI and ATP producing activities were reduced after global ischaemia, with or without reperfusion. There was a small gain in mitochondrial Ca²⁺ after ischaemia, and a large gain upon reperfusion. 5 6-OHDA pretreatment provided some protection against the effects of ischaemia and reperfusion on mitochondrial function and on peak developed tension. 6 It was concluded that chemical sympathectomy with 6-OHDA does not duplicate the effect of prolonged β-adrenoceptor blockade in protecting mitochondrial function against the deleterious effects of ischaemia and reperfusion.