Capping Structures at the 5′-Terminus of Polyadenylated Ribonucleic Acid in Avena Coleoptiles

Abstract
Evidence is presented for the occurrence of 5''-terminal capping structures in the polyadenylated RNA of oat (A. sativa) coleoptiles. These structures are composed of an inverted terminal nucleoside containing the modified base 7-methylguanine which is joined 5'' to 5'' with a second (penultimate) nucleoside by 3 phosphate groups in 2 pyrophosphate linkages. The penultimate nucleoside is joined to the remainder of the RNA molecule by a conventional 3'',5'' phosphodiester bond. A significant difference between the cap structures of oat coleoptile RNA and those of previously described higher eukaryotic cellular mRNA is the lack of ribose methylations in the penultimate nucleosides of the plant RNA.