Urinary Testosterone: A Method Utilizing Column Chromatography
- 1 March 1965
- journal article
- research article
- Published by The Endocrine Society in Journal of Clinical Endocrinology & Metabolism
- Vol. 25 (3), 315-321
- https://doi.org/10.1210/jcem-25-3-315
Abstract
A new modification of Allen''s color reaction, with certain advantages over other methods reported, is described for the determination of urinary testosterone. Isolation and good recovery are achieved by using column chromatography only. The location of testosterone in the chromatographic fractions was verified using 1, 2-H3-testosterone. This color reaction is shown to be sensitive, specific and reproducible. This process has been used to establish the amount of testosterone in the urine of a variety of subjects.Keywords
This publication has 3 references indexed in Scilit:
- Urinary C19Steroids in Two Girls with Precocious Sexual HairJournal of Clinical Endocrinology & Metabolism, 1963
- Isolation, Identification and Quantitation of Testosterone in the Urine of Normal Adults and in Patients with Endocrine DisordersJournal of Clinical Endocrinology & Metabolism, 1963
- Familial Male Sexual Precocity: Report of the Eleventh Kindred Found, with Observations on Blood Group Linkage and Urinary C19-Steroid ExcretionJournal of Clinical Endocrinology & Metabolism, 1962