Determination of DI- and Tributyltin in Sediment and Microbial Biofilms Using Acidified Methanol Extraction, Sodium Borohydride Derivatization and Gas Chromatography with Flame Photometric Detection

Abstract
A method for the relatively rapid determination of di- and tributyltin species in sediment and microbial biofilms is presented. Di- and tributyltin species were detected in Chesapeake Bay sediments using this method. Microbial biofilms grown on glass slides exhibited substantial accumulation of tributyltin species from solution. This method should have useful application to sediment analysis of di- and tributyltin species and for studies on the accumulation and fate of tributyltin in microbial biofilms.

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