Amino Acid Composition and Some Immunological Properties of a Minor Ribonuclease from Bovine Seminal Vesicles

Abstract

1) Amino acid composition and amino- and carboxyl-terminal amino acids of a ribonuclease (RNase Vs₂) having molecular weight of 13, 000 from bovine seminal vesicles were determined. 2) Using antisera against bovine pancreatic RNase A [EC 2.7.7.16] and two RNases from bovine seminal vesicles, RNase Vs₁ (major component), and RNase Vs₂ (minor component), it was found that two seminal RNases were immunologically indistinguishable but they were distinguishable from bovine pancreatic RNase so far as checked by Ouchterlony's double diffusion test.