INVESTIGATION OF ENERGY MIGRATION BETWEEN NATIVE CHLOROPHYLL FORMS AT -196DEGREESC BY METHOD OF SENSITIZED FLUORESCENCE

Abstract

Mathematical decomposition of fluorescence spectra of chloroplasts [bean] and cells measured under monochromatic excitation in the regions of absorption maxima of the native forms allowed calculation of the efficiency of energy migration between forms. Eighty to 100% of absorbed quanta flow to the .**GRAPHIC**. form (bean) in photosystem 1, whereas in photosystem 2 the .**GRAPHIC**. forms are terminal energy acceptors. Efficient backward energy transfer between the .**GRAPHIC**. forms and the existence of energy migration pathways passing 1 or 2 steps of the energy stairway formed by the energy levels of the chlorophyll forms are found. Chlorophyll b is not an intermediate link in the process of energy transfer from carotenoids to chlorophyll a. The intrinsic fluorescence quantum yields of the native forms are calculated.