Studies on the bipolar argECBH operon of E. coli: Characterization of restriction endonuclease fragments obtained from λdargECBH transducing phages and a ColE1 argECBH plasmid
- 1 January 1977
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 151 (2), 161-168
- https://doi.org/10.1007/bf00338690
Abstract
The isolation of a new type of λ transducing phage carrying the bipolar argECBH operon of E. coli K12 is described. The argECBH segment is inserted in the phage in a direction which is opposite from that of previously isolated argECBH-carrying phages. A colE1 argECBH plasmid has been constructed. DNA fragments resulting from digestion of these genetic elements with Eco RI and HindIII restriction enzymes have been characterized by agarose gel electrophoresis and electron microscopy, including heteroduplex analysis. Two fragments are of special significance for studies on the control of arginine synthesis, one of length 9.8 kilobases carrying the whole argECBH region, the other of length 2 kilobases carrying most or all of the control region between argE and argC.Keywords
This publication has 20 references indexed in Scilit:
- Recalibrated linkage map of Escherichia coli K-12.1976
- Isolation and characterization of λdargECBH transducing phages and heteroduplex analysis of the argECBH clusterMolecular Genetics and Genomics, 1976
- The argECBH-bearing EcoRI segment of the Escherichia coli chromosomeBiochemical and Biophysical Research Communications, 1975
- Studies on the cleavage of bacteriophage lambda DNA with EcoRI restriction endonucleaseJournal of Molecular Biology, 1975
- Arginine gene duplications in recombination proficient and deficient strains of Escherichia coli K 12Molecular Genetics and Genomics, 1974
- Plasmid ColE1 as a Molecular Vehicle for Cloning and Amplification of DNAProceedings of the National Academy of Sciences, 1974
- Pedigrees of some mutant strains of Escherichia coli K-12.1972
- Control regions within the argECBH gene cluster of Escherichia coli K12Molecular Genetics and Genomics, 1972
- Messenger RNA from arginine and phosphoenolpyruvate carboxylase genes in arg R+ and arg R− strains of E. coli K‐12FEBS Letters, 1971
- Selecting Bacterial Mutants by the Penicillin MethodScience, 1960