Purification and partial characterization of glyoxalase I from a higher plant Brassica juncea
- 6 May 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 282 (2), 277-280
- https://doi.org/10.1016/0014-5793(91)80494-n
Abstract
The glyoxalase I was purified from Brassica juncea by affinity chromatography on S-hexyl GSH sepharose 4B, Homogeneity of the protein was confirmed electrophoretically by a silver stained gel. Activity staining on a native starch gel also showed a single band. The effect of glutathione, methylglyoxal, and pH on enzyme kinetics was studied. Magnesium was found to stimulate the enzyme activity.Keywords
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