Avian hepatitis E virus infection and possible associated clinical disease in broiler breeder flocks in Hungary
Open Access
- 16 September 2008
- journal article
- research article
- Published by Taylor & Francis in Avian Pathology
- Vol. 37 (5), 527-535
- https://doi.org/10.1080/03079450802356946
Abstract
In broiler breeder flocks in one broiler integration in Hungary, a new syndrome appeared in January 2005 with initially four successive post-peak flocks experiencing significant decreases in egg production. Clinically birds became depressed and there was a small increase in the mortality rate. Postmortem examinations revealed enlarged livers in up to 19% of birds dying, and enlarged spleens in some. Also observed were birds with either clotted blood or serosanguineous fluid in the abdomen and subcapsular haemorrhages of the liver. Histopathology and polymerase chain reaction excluded tumours and the presence of common tumour-associated viruses. Chronic bacterial infections (especially causing hepatitis, peritonitis and airsacculitis) were common but many enlarged livers had no obvious bacterial involvement. After a 9-month period during which a majority of flocks became affected, no newly affected flocks occurred. Investigations showed that all tested affected flocks were seropositive in the big liver and spleen (BLS) Agar Gel Immunodiffusion test. Subsequent flocks without post-peak egg-production drops were shown to be seronegative in the BLS AGID test, as were all the parent flocks contributing to the affected flocks. Liver samples and cloacal swabs were positive by polymerase chain reaction (aHEV helicase target), and calicivirus-like particles were demonstrated in bile samples from affected birds. These observations are similar to hepatitis-splenomegaly syndrome as described in North America and BLS syndrome as described in Australia. Histopathological features were a non-specific chronic hepatitis similar to those described in BLS and hepatitis-splenomegaly syndrome. Immunohistochemistry using a BLS-specific monoclonal antibody confirmed the presence of avian hepatitis E virus antigen in livers and spleen.Keywords
This publication has 16 references indexed in Scilit:
- Avian hepatitis E virus in an outbreak of hepatitis–splenomegaly syndrome and fatty liver haemorrhage syndrome in two flaxseed-fed layer flocks in OntarioAvian Pathology, 2006
- Systematic Pathogenesis and Replication of Avian Hepatitis E Virus in Specific-Pathogen-Free Adult ChickensJournal of Virology, 2005
- Heterogeneity and Seroprevalence of a Newly Identified Avian Hepatitis E Virus from Chickens in the United StatesJournal of Clinical Microbiology, 2002
- The putative capsid protein of the newly identified avian hepatitis E virus shares antigenic epitopes with that of swine and human hepatitis E viruses and chicken big liver and spleen disease virusJournal of General Virology, 2002
- A low incidence of histiocytic sarcomatosis associated with infection of chickens with the HPRS-103 strain of subgroup J avian leukosis virus.Avian Diseases, 1997
- Replication of non‐defective reticuloendotheliosis viruses in the avian embryo assayed by PCR and immunofluorescenceAvian Pathology, 1997
- An antigen detection immunoassay for big liver and spleen disease agentVeterinary Microbiology, 1995
- The experimental production of big liver and spleen disease in broiler breeder hensAustralian Veterinary Journal, 1994
- Polymerase chain reaction for differentiation between pathogenic and non-pathogenic serotype 1 Marek's disease viruses (MDV) and vaccine viruses of MDV-serotypes 2 and 3Journal of Virological Methods, 1992
- Big liver and spleen disease of broiler breedersAvian Pathology, 1990