In Vitro Adhesion of Uropathogenic Escherichia coli to Human Periurethral Cells
- 1 June 1980
- journal article
- research article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 28 (3), 972-980
- https://doi.org/10.1128/iai.28.3.972-980.1980
Abstract
The in vitro adhesion of 3 uropathogenic strains of E. coli to epithelial cells from the periurethral area (area surrounding the urethral orifice) of women with and without a history of recurrent urinary tract infections was investigated. All strains showed a specific mannose-resistant hemagglutination restricted to human erythrocytes. Since only a few hundred periurethral cells were used in each test, gentle methods were required. Optimal results were obtained with bacteria grown for 16 h at 37.degree. C in nutrient broth without shaking. The binding of bacteria seemed irreversible under the conditions studied, since repeated washings of the epithelial cells after incubation did not decrease the number of adhering bacteria. Chloramphenicol was used to control the number of added bacteria in the incubation system. A difference in the adhesive capacity of periurethral cells of infection-prone and healthy individuals was most evident at concentrations of 2.5 .times. 109 bacteria/ml. EM studies indicated that pili mediated the adhesion. Adhesion was correlated with the mannose-resistant hemagglutination of human erythrocytes, indicating that the pili were not type 1 pili. Day-to-day variations in the adhesiveness of the bacteria were reduced by selecting well-adhering bacteria with the aid of in vitro passage on periurethral cells or human erythrocytes and by exclusion of bacteria with low hemagglutination ability.This publication has 21 references indexed in Scilit:
- The Fimbrial and Non-Fimbrial Haemagglutinins of Escherichia ColiJournal of Medical Microbiology, 1979
- BACTERIAL ADHERENCE TO PERIURETHRAL EPITHELIAL CELLS IN GIRLS PRONE TO URINARY-TRACT INFECTIONSThe Lancet, 1978
- Hemagglutination of human group A erythrocytes by enterotoxigenic Escherichia coli isolated from adults with diarrhea: correlation with colonization factor.1977
- Haemagglutinating and Adhesive Properties Associated with the K99 Antigen of Bovine Strains of Escherichia coliJournal of General Microbiology, 1976
- Variable adherence to normal human urinary-tract epithelial cells of Escherichia coli strains associated with various forms of urinary-tract infection.1976
- The Role of Vaginal Colonization with Enterobacteriaceae in Recurrent Urinary InfectionsJournal of Urology, 1975
- The Association of K88 Antigen with Haemagglutinating Activity in Porcine Strains of Escherichia coliJournal of General Microbiology, 1974
- Further Observations On Escherichia Coli Enterotoxins With Particular Regard To Those Produced By Atypical Piglet Strains And By Calf And Lamb Strains: The Transmissible Nature Of These Enterotoxins And Of A K Antigen Possessed By Calf And Lamb StrainsJournal of Medical Microbiology, 1972
- Antibiotic sensitivity testing. Report of an international collaborative study.1971
- THE STRUCTURE, FUNCTION, SYNTHESIS AND GENETIC CONTROL OF BACTERIAL PILI AND A MOLECULAR MODEL FOR DNA AND RNA TRANSPORT IN GRAM NEGATIVE BACTERIA*Transactions of the New York Academy of Sciences, 1965