The Chemical Estimation of Acyl Glucuronides and Its Application to Studies on the Metabolism of Benzoate and Salicylate in Man

Abstract

Acyl glucuronides react with hydroxylamine to form hydroxamic acids. This reaction was used for their estimation, purification, and identification. Benzoate, salicylate, and probenecid elicited the urinary excretion of their respective acyl glucuronides when ingested orally by a normal subject. Doses of sodium benzoate were quantitatively recovered as urinary hippurate and benzoyl glucuronide. At least 90% of various doses of salicylate were recovered as urinary salicylurate, salicyl acyl glucuronide, and salicyl phenolic glucuronide. Characteristic differences in the urinary excretory patterns of salicyl acyl glucuronide and salicyl phenolic glucuronide were described.