An approach towards surface imprinting using the enzyme ribonuclease A
- 1 January 1995
- journal article
- research article
- Published by Wiley in Journal of Molecular Recognition
- Vol. 8 (1-2), 35-39
- https://doi.org/10.1002/jmr.300080106
Abstract
An Adsorbent showing enhanced selectivity for the enzyme RNase A was prepared by a surface imprinting procedure based on metal coordination. A metal chelating monomer, N-(4-vinyl)-benzyl iminodiacetic acid, was polymerized onto methacrylate-derivated silica particles in the presence of RNase A and metal ions. Lysozyme and RNase A were separated on the adsorbent used as stationary phase in high-performance liquid chromatography.Keywords
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