Zinc is required for folding and binding of a single zinc finger to DNA
- 25 February 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 279 (2), 289-294
- https://doi.org/10.1016/0014-5793(91)80170-8
Abstract
A synthetic peptide corresponding to zinc finger 31 of the Xenopus protein adopts a folded conformation in the presence of zinc. The same peptide in the absence of zinc is not folded in a stable tertiary conformation, as determined by NMR. Binding experiments have shown that the peptide binds non-specifically to DNA only in the presence of zinc. Moreover, competive DNA binding experiments indicate interaction with 3.9 ± base pairsKeywords
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