Mitotic and pheromone-specific intrinsic polarization cues interfere with gradient sensing in Saccharomyces cerevisiae
- 24 March 2020
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 117 (12), 6580-6589
- https://doi.org/10.1073/pnas.1912505117
Abstract
Polarity decisions are central to many processes, including mitosis and chemotropism. In Saccharomyces cerevisiae, budding and mating projection (MP) formation use an overlapping system of cortical landmarks that converges on the small G protein Cdc42. However, pheromone-gradient sensing must override the Rsr1-dependent internal polarity cues used for budding. Using this model system, we asked what happens when intrinsic and extrinsic spatial cues are not aligned. Is there competition, or collaboration? By live-cell microscopy and microfluidics techniques, we uncovered three previously overlooked features of this signaling system. First, the cytokinesis-associated polarization patch serves as a polarity landmark independently of all known cues. Second, the Rax1-Rax2 complex functions as a pheromone-promoted polarity cue in the distal pole of the cells. Third, internal cues remain active during pheromone-gradient tracking and can interfere with this process, biasing the location of MPs. Yeast defective in internal-cue utilization align significantly better than wild type with artificially generated pheromone gradients.Keywords
Funding Information
- HHS | NIH | National Institute of General Medical Sciences (1R01GM097479-01)
- MINCyT | ANPCyT | Fondo para la Investigación Científica y Tecnológica (PICT2013-2210, PICT2015-3824, PICT2016-0949)
This publication has 76 references indexed in Scilit:
- Cell Polarization and Cytokinesis in Budding YeastGenetics, 2012
- Polarization of the Yeast Pheromone Receptor Requires Its Internalization but Not Actin-dependent SecretionMolecular Biology of the Cell, 2010
- Neutrophils Establish Rapid and Robust WAVE Complex Polarity in an Actin-Dependent FashionCurrent Biology, 2009
- Robust Spatial Sensing of Mating Pheromone Gradients by Yeast CellsPLOS ONE, 2008
- Symmetry-Breaking Polarization Driven by a Cdc42p GEF-PAK ComplexCurrent Biology, 2008
- Adjacent positioning of cellular structures enabled by a Cdc42 GTPase-activating protein–mediated zone of inhibitionThe Journal of cell biology, 2007
- Distinct Domains of Yeast Cortical Tag Proteins Bud8p and Bud9p Confer Polar Localization and FunctionalityMolecular Biology of the Cell, 2007
- Polarity reveals intrinsic cell chiralityProceedings of the National Academy of Sciences, 2007
- Central Roles of Small GTPases in the Development of Cell Polarity in Yeast and BeyondMicrobiology and Molecular Biology Reviews, 2007
- Control of yeast cell type by the mating type locusJournal of Molecular Biology, 1981