Sodium Butyrate Stimulates Somatostatin Production by Cultured Cells*
- 1 June 1984
- journal article
- research article
- Published by The Endocrine Society in Endocrinology
- Vol. 114 (6), 1990-1994
- https://doi.org/10.1210/endo-114-6-1990
Abstract
Expression of the neuropeptide SRIF [somatostatin] can be enhanced by sodium butyrate, a known modulator of gene transcription. Two different cell lines, RIN cells (derived from a rat pancreatic islet tumor) as well as human cervical carcinoma HeLa cells, showed basal secretion of SRIF immunoreactive material that was induced between 5- and 15-fold by sodium butyrate. The induction was dose and time dependent, fully reversible, and specific for sodium butyrate. Analysis of the induced mRNA by Northern blot hybridization using a SRIF specific complementary DNA probe revealed a 12-fold increase in the level of SRIF mRNA caused by butyrate treatment. Sodium butyrate may be a useful tool for studying the regulation of SRIF gene expression.This publication has 20 references indexed in Scilit:
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