New Fluorimetric Detection Method of Corticosteroids after High-Performance Liquid Chromatography Using Post-Column Derivatization with Glycinamide

Abstract

Some biological corticosteroids were detected by a new fluorimetric method using post-column derivatization with glycinamide. Urinary corticosteroids which are hydrolyzed and extracted from sample fluid are detected by the reaction with glycinamide in the presence of hexacyanoferrate (III) in weakly alkaline media of borate buffer solution of pH 9.8 at 90 [ddot]C for 5 min. This method is highly sensitive and specific for some corticosteroids and the reaction is performed under mild condition, compared with the fluorimetric method using the reaction with sulfuric acid, so that this method can be used for fractional assay of urinary corticosteroids of patients for a steroid abnormal metabolism.