The relationship of serum estradiol and progesterone concentrations to the enzyme immunoassay measurements of urinary estrone conjugates and immunoreactive pregnanediol‐3‐glucuronide in Macaca mulatta

Abstract

Paired urine and serum samples from four conceptive and six nonconceptive ovarian cycles of seven adult Macaca mullatta were analyzed by radioimmunoassay (RIA) for circulating estradiol (E₂) and progesterone (Po), and urinary estrone conjugates (E₁C) and immunoreactive preganediol-3-glucuronide (iPDG) using enzyme immunoassay (EIA). Nonconceptive cycles exhibited a fivefold increase in urinary E₁C and serum E₂ levels from follicular phase levels to the preovulatory peak. Linear correlation between urinary E₁C and serum E₂ nonconceptive cycle hormone levels was significant (P 1C levels and serum E₂ measurements had a correlation coefficient that was significant (PP = 0.05, r = 0.63, and PMacaca mulatta.