Curtain Electrophoretic Separation of Phenyl Acetate Hydrolysing Esterases of Human Serum. Demonstration of an EDTA-Dependent Enzyme.

Abstract

By repeated continuous flow electrophoresis of human serum in [image]/150 phosphate pH 7.9, two distinct phenyl acetate hydrolyzing esterases were purified 120 and 850 times. One esterase was heat-labile and inactivated by EDTA (corresponding to A-esterase), while the other was heat stable and required EDTA for activity. This EDTA-dependent enzyme has not been described heretofore. The methods for purification and assay are described and the results discussed.