Nucleotide sequence of a spectinomycin adenyltransferase AAD(9) determinant from Staphylococcus aureus and its relationship to AAD(3″) (9)
- 1 June 1985
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 200 (1), 33-39
- https://doi.org/10.1007/bf00383309
Abstract
The nucleotide sequence of the spc determinant of the Staphylococcus aureus transposon Tn554 has been determined. This gene encodes a spectinomycin adenyltransferase, AAD(9), that mediates resistance to spectinomycin but not to streptomycin. The sequence predicts a 260 amino acid protein of molecular weight 28,943. A spectinomycin-sensitive mutant (spc-1) contains a G→A transition resulting in substitution of threonine (ACA) for alanine (GCA) at residue 165. The predicted amino acid sequence is 36% homologous to that of a widely distributed, gramnegative streptomycin/spectinomycin adenyltransferase, AAD(3″) (9), specified by the aadA determinant (Holingshead and Vapnek 1985).This publication has 32 references indexed in Scilit:
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