Anchorage-independent cell growth-inhibiting factor(s) from normal rat liver and ascites hepatoma
- 1 October 1986
- journal article
- research article
- Published by Wiley in Cell Biology International Reports
- Vol. 10 (10), 813-820
- https://doi.org/10.1016/0309-1651(86)90151-7
Abstract
Acid/ethanol extracts of normal rat liver and rat ascites hepatoma cells (AH 130) were found to inhibit the epidermal growth factor (EGF)-dependent colony formation of Balb/c 3T3 cells. The extracts did not inhibit the growth of the 3T3 cells in monolayer culture. The inhibitory activities were heat- and acid-stable and were not inactivated by the treatment with pronase, RNase and DNase. Upon ultrafiltration, a considerable fraction of the inhibitory activities were found in the fraction corresponding to the molecular size ranging from 3.5 to 10 Kd, in which no appreciable TGF-.beta. activity was detected.This publication has 15 references indexed in Scilit:
- IDENTIFICATION OF ENDOGENOUS INHIBITORY GROWTH-FACTORS FROM A HUMAN-COLON CARCINOMA CELL-LINE1985
- Type beta transforming growth factor: a bifunctional regulator of cellular growth.Proceedings of the National Academy of Sciences, 1985
- Identification of a tumor inhibitory factor in rat ascites fluidBiochemical and Biophysical Research Communications, 1984
- Human leukemic cells contain transforming growth factorCancer Letters, 1983
- Cellular responsiveness to growth factors correlates with a cell's ability to express the transformed phenotypeCell, 1983
- New class of transforming growth factors potentiated by epidermal growth factor: isolation from non-neoplastic tissues.Proceedings of the National Academy of Sciences, 1981
- Transforming growth factors: isolation of polypeptides from virally and chemically transformed cells by acid/ethanol extraction.Proceedings of the National Academy of Sciences, 1980
- Correlation of patterns of anchorage-independent growth with in vivo behavior of cells from a murine fibrosarcoma.Proceedings of the National Academy of Sciences, 1980
- Lymphocyte and Fibroblast Chalones: Some Chemical PropertiesScience, 1977
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951