Geometry of the First Step in the Action of Ribonuclease-A
- 1 January 1972
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 69 (1), 115-118
- https://doi.org/10.1073/pnas.69.1.115
Abstract
Advantage was taken of the reversibility of the first step of ribonuclease-A action to synthesize the dinucleoside phosphorothioate Up(S)C from the crystalline isomer of uridine 2',3'-cyclic phosphorothioate [Up(S)] and cytidine. Cyclic phosphorothioate was then reformed from Up(S)C by a nonenzymic reaction known to proceed by an in-line mechanism. The geometry of the enzymic reaction was determined to be in-line by a comparison of the Up(S) product with the Up(S) originally used. By the principle of microscopic reversibility, the geometry of the first step in the action of ribonuclease-A is shown to be in-line.Keywords
This publication has 14 references indexed in Scilit:
- Nucleoside phosphorothioatesJournal of the American Chemical Society, 1970
- Synthesis of 3′–5′ dinucleotides with RNase T1Biochemical and Biophysical Research Communications, 1970
- RibonucleasesAnnual Review of Biochemistry, 1969
- Enzymatic synthesis of oligoguanylic acids containing 2′–5′ phosphodiester linkagesBiochemical and Biophysical Research Communications, 1969
- ON THE MECHANISM OF RIBONUCLEASE ACTIONProceedings of the National Academy of Sciences, 1969
- Uridine 2′,3′‐O,O‐cyclophosphorothioate as substrate for pancreatic ribonuclease (I)FEBS Letters, 1968
- Facilitated Proton Transfer in Enzyme CatalysisScience, 1968
- Relaxation Spectrometry of Biological SystemsAdvances in protein chemistry, 1968
- Zum Mechanismus der Ribonuclease‐ReaktionEuropean Journal of Biochemistry, 1967
- The Active Site and Mechanism of Action of Bovine Pancreatic RibonucleaseNature, 1961