An Osmotic Barrier for Inorganic Phosphate in Chick Embryo Cells and its Stability during the Latent and Release Periods of Infection by Vesicular Stomatitis Virus

Abstract

The paths of phosphate transfer in normal chick embryo cells and in cells infected with vesicular stomatitis virus (VSV) were compared. Data are presented indicating the existence of an osmotic barrier close to the chick embryo cell wall, which appears to regulate the exchange of inorganic orthophosphate by means of a transport mechanism. VSV has little effect on the restriction at the cell surface, indicating that penetration and release of virus can result in only a very limited lysis of the cell wall, and that the passage of virus does not alter an enzyme-dependent transport system.