Bioaccumulation, Retention, and Depuration of Enteric Viruses by Crassostrea virginica and Crassostrea ariakensis Oysters
- 15 November 2008
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 74 (22), 6825-6831
- https://doi.org/10.1128/aem.01000-08
Abstract
Crassostrea ariakensis oysters are under review for introduction into the Chesapeake Bay. However, the human health implications of the introduction have not been fully addressed. This study evaluated rates of bioaccumulation, retention, and depuration of viruses by Crassostrea virginica and C. ariakensis when the two oyster species were maintained in separate tanks containing synthetic seawater of various salinities (8, 12, or 20 ppt). Oyster bioaccumulation tanks were seeded with 10 3 PFU/ml of hepatitis A virus (HAV), poliovirus, male-specific bacteriophage (MS2), and murine norovirus 1 (MNV-1) and 10 3 PCR units/ml of human norovirus (NoV). After 24 h, depuration commenced as oysters ( n = 255) were placed in pathogen-free seawater under continuous filtration. Oysters ( n = 6) were sampled weekly for 1 month from each tank. Viral RNA was recovered using a modified proteinase K, guanidine, and glassmilk method and analyzed by quantitative reverse transcription-PCR. The odds of C. ariakensis oysters harboring NoV, MNV-1, or HAV were statistically greater than the odds of C. virginica oysters harboring the same viruses (MNV-1 odds ratio [OR], 4.5; P = 0.01; NoV OR, 8.4; P < 0.001; HAV OR, 11.4; P < 0.001). Unlike C. virginica, C. ariakensis bioaccumulated and retained NoV, MNV-1, and HAV for 1 month at all salinities. Additionally, the odds of an oyster testing positive for NoV was 25.5 times greater ( P < 0.001) when the oyster also tested positive for MNV-1. This research helps assess the threat of C. ariakensis as a vehicle for viral pathogens due to the consumption of raw oysters and validates the role for MNV-1 as a surrogate for NoV.Keywords
This publication has 30 references indexed in Scilit:
- Evaluation of Murine Norovirus, Feline Calicivirus, Poliovirus, and MS2 as Surrogates for Human Norovirus in a Model of Viral Persistence in Surface Water and GroundwaterApplied and Environmental Microbiology, 2008
- Persistence of Caliciviruses in Artificially Contaminated Oysters during DepurationApplied and Environmental Microbiology, 2007
- Norovirus mixed infection in an oyster-associated outbreak: an opportunity for recombinationArchiv für die gesamte Virusforschung, 2007
- Norovirus binds to blood group A-like antigens in oyster gastrointestinal cellsLetters in Applied Microbiology, 2006
- Detection of Multiple Noroviruses Associated with an International Gastroenteritis Outbreak Linked to Oyster ConsumptionJournal of Clinical Microbiology, 2006
- Murine Norovirus: a Model System To Study Norovirus Biology and PathogenesisJournal of Virology, 2006
- Recovery, Bioaccumulation, and Inactivation of Human Waterborne Pathogens by the Chesapeake Bay Nonnative Oyster, Crassostrea ariakensisApplied and Environmental Microbiology, 2006
- Use of Rotavirus Virus-Like Particles as Surrogates To Evaluate Virus Persistence in ShellfishApplied and Environmental Microbiology, 2005
- Replication of Norovirus in Cell Culture Reveals a Tropism for Dendritic Cells and MacrophagesPLoS Biology, 2004
- Development of Methods To Detect “Norwalk-Like Viruses” (NLVs) and Hepatitis A Virus in Delicatessen Foods: Application to a Food-Borne NLV OutbreakApplied and Environmental Microbiology, 2000