MOLECULAR PROPERTIES OF T LYMPHOMA IMMUNOGLOBULIN
- 1 August 1977
- journal article
- Published by Wiley in International Journal of Immunogenetics
- Vol. 4 (4), 233-248
- https://doi.org/10.1111/j.1744-313x.1977.tb00906.x
Abstract
Immunoglobulin (Ig) released into the medium by monoclonal continuously cultured murine T lymphoma cells of the lines WEHI-22 and WEHI-7 was isolated by serological precipitation or solid-phase immunoadsorption techniques. The intact immunoglobulin had an electrophoretic mobility on sodium dodecyl-sulphate (SDS) containing polyacrylamide gels comparable to that of IgG (mass 150,000). This mobility was significantly faster than that of ‘7S’ IgM of murine B lymphocyte surfaces. The T lymphoma immunoglobulin consisted of a pair of heavy chains linked by disulphide bonds and light chains non-covalently bound to the heavy chains. The isolated heavy chains migrated slightly faster than the μ chains of MOPC 104E IgM. Some, but not all, antisera directed against μ chains of normal mouse serum IgM bound T lymphoma immunoglobulin apparently via a cross-reaction localized to the Fd fragment. These data indicate that immunoglobulin of T lymphoma cells and, presumably normal T lymphocytes, represents an immunoglobulin isotype which is distinct from those immunoglobulins found on the B cell surface.Keywords
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