In Vivo and In Vitro Localization of Leishmania within Macrophage Phagolysosomes: Use of Colloidal Gold as a Lysosomal Label1

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Abstract
The interaction of Leishmania with lysosomes within macrophages in vivo was investigated. Lysosomes labeled with colloidal gold in vivo fused with phagocytic vacuoles containing Leishmania amastigotes within the macrophages of infected footpad tissue of BALB/c mice. This localization of Leishmania within macrophage phagolysosomes in vivo is the 1st confirmation for any obligate intracellular protozoon that parasite-lysosome interactions in vitro occur in vivo.