Base Composition of Deoxyribonucleic Acid of the Temperature-Sensitive Kanamycin-Resistant R Factor, R ts 1

Abstract
The buoyant density of deoxyribonucleic acid (DNA) from the temperature-sensitive R factor, R ts 1, was determined by CsCl density-gradient centrifugation. R ts 1 was found to consist of a single species of DNA of density 1.705 g/cm 3 , which corresponds to a base composition of 45% guanine plus cytosine. This value is distinct from the densities previously reported for other R factors, suggesting that R ts 1 represents a new molecular class of R factors.