The binding of [3H] pargyline to rat liver mitochondrial monoamine oxidase
- 1 September 1980
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Pharmacy and Pharmacology
- Vol. 32 (1), 49-54
- https://doi.org/10.1111/j.2042-7158.1980.tb12844.x
Abstract
The synthesis and purification of tritium labelled N-desmethylpargyline and pargyline are described. The suitability of these irreversible suicide inhibitors of monoamine oxidase (MAO) as ligands in binding studies to rat liver mitochondrial MAO has been evaluated. [3 H] Pargyline was found to be more satisfactory than its N-desmethyl analogue because of its greater potency and lower proportion of non-specific binding. The binding of pargyline reached saturation when about 31 pmol mg protein−1 was bound. It was not possible to explain the time course of the binding by either simple first or second-order kinetics. [3 H]-Pargyline is a potentially valuable ligand for the estimation of the concentration of MAO active centres without the need to remove the enzyme from the mitochondrial membrane.This publication has 18 references indexed in Scilit:
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