Attomole Detection of Mesophilic DNA Polymerase Products by Nanoparticle-Enhanced Surface Plasmon Resonance Imaging on Glassified Gold Surfaces
- 21 June 2010
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 132 (27), 9265-9267
- https://doi.org/10.1021/ja103043p
Abstract
DNA microarrays are invaluable tools for biosensing applications such as diagnostic detection of DNA and analysis of gene expression. Surface plasmon resonance imaging can detect unlabeled oligonucleotide targets adsorbed to the array elements. The variety of biosensing applications can be expanded by enzymatic manipulation of DNA microarray elements, and the sensitivity of detection can be enhanced with the use of oligonucleotide immobilized onto a gold nanoparticle surface. We describe a novel method that couples a template-directed polymerase extension of a surface array element with nanoparticle-enhanced detection of the reaction product. Using this technique, it is possible to see as little as 10−100 amol of polymerase product, representing as little as 0.25% of a monolayer. This sensitivity would allow for the detection of a specific DNA target that is present in low amounts in a sample and with partially unknown sequence. One application of this method would be to identify the presence of the aberrantly recombined DNA sequences, such as those found in the fragile sites of chromosomes.Keywords
This publication has 15 references indexed in Scilit:
- Attomole Microarray Detection of MicroRNAs by Nanoparticle-Amplified SPR Imaging Measurements of Surface Polyadenylation ReactionsJournal of the American Chemical Society, 2006
- Creating Advanced Multifunctional Biosensors with Surface Enzymatic TransformationsLangmuir, 2006
- Single-Nucleotide Polymorphism Genotyping by Nanoparticle-Enhanced Surface Plasmon Resonance Imaging Measurements of Surface Ligation ReactionsAnalytical Chemistry, 2006
- Nanoscale Glassification of Gold Substrates for Surface Plasmon Resonance Analysis of Protein Toxins with Supported Lipid MembranesAnalytical Chemistry, 2005
- Enzymatically Amplified Surface Plasmon Resonance Imaging Detection of DNA by Exonuclease III Digestion of DNA MicroarraysAnalytical Chemistry, 2005
- Enzymatically Amplified Surface Plasmon Resonance Imaging Method Using RNase H and RNA Microarrays for the Ultrasensitive Detection of Nucleic AcidsAnalytical Chemistry, 2004
- Direct Detection of Genomic DNA by Enzymatically Amplified SPR Imaging Measurements of RNA MicroarraysJournal of the American Chemical Society, 2004
- Surface Plasmon Resonance Imaging as a Tool to Monitor Biomolecular Interactions in an Array Based FormatApplied Spectroscopy, 2003
- Surface Plasmon Resonance Imaging Measurements of DNA and RNA Hybridization Adsorption onto DNA MicroarraysAnalytical Chemistry, 2000
- Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA MicroarrayScience, 1995