Forty-six Rhizoctonia isolates from greenhouse-grown bedding plants and field-grown nonbedding plants were evaluated for pathogenicity on 6 bedding plant hosts. More isolates from bedding plants were pathogenic and generally more virulent than those from nonbedding plants. Hosts differed significantly in their susceptibility to Rhizoctonia damping-off. Impatiens [Impatiens wallerana] was the most susceptible; cabbage [Brassica oleracea var. capitata] and celosia [Celosia argentea] were somewhat less susceptible; ageratum [Ageratum houstonianum] was moderately susceptible; and tomatoes [Lycopersicon esculentum] and peppers [Capsicum frutescens] were the least susceptible hosts. All bedding plant isolates were R. solani, anastomosis group 4 (AG-4). Some nonbedding plant isolates were other fungi, and those that were R. solani were of several anastomosis groups.