Cholinergic metabolism and synapse formation by a rat nerve cell line.
- 1 June 1977
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 74 (6), 2579-2583
- https://doi.org/10.1073/pnas.74.6.2579
Abstract
The PC12 clone of a rat pheochromocytoma was able to synthesize acetylcholine. The amount of acetylcholine synthesized, and the specific activity of choline O-acetyltransferase (acetyl-CoA:choline O-acetyltransferase, EC 2.3.1.6), varied as a function of the culture growth curve and was dependent on cell density. The specific activity of choline acetyltransferase was increased by nerve growth factor, growth-conditioned medium from a variety of cell types and cyclic AMP. The PC12 cell line was able to form cholinergic synapses with a clonal cell line of skeletal muscle [rat] origin.This publication has 35 references indexed in Scilit:
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