New selective media forpseudomonasstrains producing fluorescent pigment

Abstract

Four selective media for Pseudomonas strains producing fluorescent pigment (P-l medium), Pseudomonas putida strains (P-2 medum) and Pseudomonas fluorescens strains (P-3 and P-4 media) were proposed on the basis of the assimilation of carbon sources by the strains. One hundred and three strains of Pseudomonas species producing fluorescent pigment were isolated from soils and plant roots, and used for the evaluation of these selective media. Most of the strains identified as P. putida produced fluorescent pigment on P-2 medium unlike the others, suggesting that the medium can be used as a selective medium for P. putida strains. Most of the strains of P. fluorescens and a small proportion of those of P. putida produced fluorescent pigment on the P-3 or P-4 medium. As the number of P. putida which produced fluorescent pigment on the P-3 and P-4 media was far smaller than the number of P. fluorescens on P-3 and P-4 media, the P-3 and P-4 media were used as selective media for P. fluorescens. The distribution of P. putida and P. fluorescens strains in 6 soils and on 6 plant roots was studied using these selective media. Pseudomonas putida and P. fluorescens were distributed widely but unevenly through the soils and plant roots.P. putida.