Investigation of the mating system of Pseudomonas aeruginosa strain 1: IV. Mapping of distal markers

Abstract

Thirteen selected distal markers have been mapped by time of entry studies and linkage analysis. They appear to fall on two distinct linkage groups and with the ten previously described there are nineteen markers on one group and four on the other. There is as yet no clear evidence that the two groups are linked, but this possibility has not been excluded.Of the thirteen markers described, six can be mapped by measuring their time of entry into suitable recipients. It should now be possible to establish the order of any further marker and to provide more accurate information for any marker transferred within 35 min from the time of mixing the cells.