p47 is a cofactor for p97-mediated membrane fusion
- 3 July 1997
- journal article
- letter
- Published by Springer Nature in Nature
- Vol. 388 (6637), 75-78
- https://doi.org/10.1038/40411
Abstract
At least two distinct ATPases, NSF and p97, are known to be involved in the heterotypic fusion of transport vesicles with their target membranes and the homotypic fusion of membrane compartments1. The NSF-mediated fusion pathway is the best characterized, many of the components having been identified and their functions analysed2,3,4,5,6,7. In contrast, none of the accessory proteins for the p97-mediated fusion pathway has been identified8,9,10. Now we have identified the first such component, a protein of relative molecular mass 47,000 (p47), which forms a tight, stoichiometric complex with cytosolic p97 (one trimer of p47 per hexamer of p97). It is essential for the p97-mediated regrowth of Golgi cisternae from mitotic Golgi fragments, a process restricted to animal cells11. As a homologue of p47 exists in budding yeast, this indicates that it might also be involved in other membrane fusion reactions catalysed by p97, such as karyogamy10.Keywords
This publication has 25 references indexed in Scilit:
- TRANSPORT VESICLE DOCKING: SNAREs and AssociatesAnnual Review of Cell and Developmental Biology, 1996
- Protein Sorting by Transport VesiclesScience, 1996
- Membrane fusion and the cell cycle: Cdc48p participates in the fusion of ER membranesCell, 1995
- An NSF-like ATPase, p97, and NSF mediate cisternal regrowth from mitotic golgi fragmentsCell, 1995
- The formation of golgi stacks from vesiculated golgi membranes requires two distinct fusion eventsCell, 1995
- Clostridial neurotoxins as tools to investigate the molecular events of neurotransmitter releaseSeminars in Cell Biology, 1994
- Vesicle fusion from yeast to manNature, 1994
- GTPases: Multifunctional Molecular Switches Regulating Vesicular TrafficAnnual Review of Biochemistry, 1994
- Implications of the SNARE hypothesis for intracellular membrane topology and dynamicsCurrent Biology, 1994
- VESICLE-MEDIATED PROTEIN SORTINGAnnual Review of Biochemistry, 1992