Enhanced binding of phospholipase-A2-modified low density lipoprotein by human adipocytes
- 1 November 1990
- journal article
- research article
- Published by Canadian Science Publishing in Biochemistry and Cell Biology
- Vol. 68 (11), 1243-1249
- https://doi.org/10.1139/o90-184
Abstract
Recognition of low density lipoprotein (LDL) by human adipocytes is not dependent on the classical LDL (apoprotein B–E) receptor. To assess whether LDL phospholipids have a role in adipocyte-LDL interactions, binding studies were carried out with human LDL modified with cobra venom phospholipase A2 (PLA2) and freshly isolated adipocytes and purified adipocyte plasma membranes prepared from surgical biopsies. LDL incubated with PLA2 showed increased monoacylphospholipid content, decreased diacylphospholipid content, and increased anodic migration on agarose gel electrophoresis. LDL cholesterol, triglyceride, and protein content remained unchanged. Typically, modification of 16 and 47% of LDL phospholipids enhanced specific binding of 125I-labelled LDL to plasma membranes progressively from 3.1 μg LDL bound/mg membrane protein (control) to 5.8 and 28.2 μg LDL bound/mg membrane protein, respectively. Nonspecific binding was not altered significantly. Excess unlabelled native LDL and high density lipoprotein (HDL3) effectively inhibited binding of PLA2-modified LDL. Freshly isolated adipocytes also showed enhanced binding and uptake of PLA2-modified LDL (0.1 vs. 0.9 μg LDL/(106 cells∙2 h), control vs. modified). The results demonstrate that alterations of LDL phospholipids significantly enhance LDL binding and suggest a regulatory role for phospholipids in lipoprotein–cell interaction. Furthermore, the results support the view that human adipose tissue may be involved in the metabolism of modified lipoproteins, in vivo.Key words: low density lipoprotein, adipocyte, phospholipase, lipoprotein receptors.This publication has 32 references indexed in Scilit:
- The scavenger cell pathway for lipoprotein degradation: Specificity of the binding site that mediates the uptake of negatively‐charged LDL by macrophagesJournal of Supramolecular Structure, 1980
- Synthesis of fatty acids and cholesterol by liver, adipose tissue and intestinal mucosa from obese and control patientsEuropean Journal of Clinical Investigation, 1979
- Low density lipoprotein binding, internalization, and degradation in human adipose cellsCanadian Journal of Biochemistry, 1979
- Role of lysine residues of plasma lipoproteins in high affinity binding to cell surface receptors on human fibroblasts.Journal of Biological Chemistry, 1978
- Characterization of the low density lipoprotein receptor in membranes prepared from human fibroblasts.Journal of Biological Chemistry, 1978
- High density lipoproteins reduce the uptake of low density lipoproteins by human endothelial cells in cultureBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1976
- Determination of free amino groups in proteins by trinitrobenzenesulfonic acidAnalytical Biochemistry, 1966
- METABOLISM OF ISOLATED FAT CELLS .I. EFFECTS OF HORMONES ON GLUCOSE METABOLISM + LIPOLYSIS1964
- THE DISTRIBUTION AND CHEMICAL COMPOSITION OF ULTRACENTRIFUGALLY SEPARATED LIPOPROTEINS IN HUMAN SERUMJCI Insight, 1955
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951