Variation in colonial morphology of Neisseria gonorrhoeae after growth on media containing antimicrobial agents.
- 1 September 1973
- journal article
- Vol. 26 (3), 248-51
Abstract
Stable colonial types 1, 2, 3, and 4 were prepared from eight strains of Neisseria gonorrhoeae. Four of the strains, termed laboratory strains, had been transferred over 100 times; three strains, termed clinical strains, were transferred only three to five times after isolation from patients, and one stabilized clinical strain was transferred purposefully 30 times after isolation from a patient. Colonial types of the three categories were grown on four media containing the following agents at the level used in diagnostic media: (i) vancomycin, colistin, and nystatin; (ii) these antibiotics plus trimethoprim lactate; (iii) trimethoprim lactate alone; and (iv) a control with no antimicrobial agents. When grown on media containing the antimicrobial agents, colonial types 1, 2, and 3 of all strains showed specific and consistent changes that precluded accurate identification of the types. In general, the colonies were smaller, more dense to transmission of light, and more granular than colonies grown on control medium. More colonies showed these type changes in the clinical strains and on media containing trimethoprim lactate. Colonies of type 4 showed little or no change. The changes in colonial morphology of types 1, 2, and 3 were pronounced enough to make colony typing difficult if the antimicrobial agents, particularly trimethoprim lactate, were present in media.This publication has 8 references indexed in Scilit:
- STUDIES ON GONOCOCCUS INFECTIONThe Journal of Experimental Medicine, 1972
- Neisseria gonorrhoeae: colonial morphology of rectal isolates.1972
- Maintenance of Virulent Gonococci in Laboratory CultureNature New Biology, 1972
- STUDIES ON GONOCOCCUS INFECTIONThe Journal of Experimental Medicine, 1971
- Transgrow, a medium for transport and growth of Neisseria gonorrhoeae and Neisseria meningitidis.1971
- Gonococci in Urethral Exudates possess a Virulence Factor lost on SubcultureNature, 1970
- Primary isolation of Neisseria gonorrhoeae on hemoglobin-free Thayer-Martin medium.1970