Simultaneous concentration of platelets and marrow cells: A simple and useful technique to obtain source cells and growth factors for regenerative medicine
- 23 January 2007
- journal article
- Published by Wiley in Wound Repair and Regeneration
- Vol. 15 (1), 156-162
- https://doi.org/10.1111/j.1524-475x.2006.00196.x
Abstract
Platelet-rich plasma (PRP) has attracted attention as a safe and cost-effective source of growth factors that stimulate cells to regenerate tissue. Bone marrow aspirate was processed with the same protocol to obtain PRP from peripheral blood. This concentrate contained condensed nucleated bone marrow cells, which are useful for regenerative medicine, as well as condensed platelets. In PRP derived from bone marrow aspirate, the density of platelets and levels of growth factors (platelet-derived growth factor and transforming growth factor-beta) were the same as in PRP derived from peripheral blood. Condensation of nucleated cells, especially small-sized cells, was confirmed. With a simple and cost-effective technique, source cells and growth factors can be obtained at the same time. This simultaneous concentration of platelets and bone marrow cells has great potential as a source of materials for regenerative medicine.Keywords
This publication has 29 references indexed in Scilit:
- Bone marrow cell transplantation in clinical perspectiveJournal of Molecular and Cellular Cardiology, 2005
- Human Primary Endothelial Cells Stimulate Human Osteoprogenitor Cell DifferentiationCellular Physiology and Biochemistry, 2004
- Adult stem cell therapy for the heartThe International Journal of Biochemistry & Cell Biology, 2004
- Effects of platelet‐rich plasma on bone healing in combination with autogenous bone and bone substitutes in critical‐size defectsClinical Oral Implants Research, 2004
- Comparative study of β-tricalcium phosphate mixed with platelet-rich plasma versus β-tricalcium phosphate, a bone substitute material in dentistryActa Veterinaria Hungarica, 2003
- Platelet‐Rich Plasma Contains High Levels of Platelet‐Derived Growth Factor and Transforming Growth Factor‐β and Modulates the Proliferation of Periodontally Related Cells In VitroThe Journal of Periodontology, 2003
- The Tetrahymena chaperonin subunit CCTη gene is coexpressed with CCTγ gene during cilia biogenesis and cell sexual reproductionFEBS Letters, 1996
- Involvement of platelets in stimulating osteogenic activityJournal of Orthopaedic Research, 1995
- Insulin-like growth factor binding protein-3 is present in the alpha- granules of plateletsEndocrinology, 1993
- Pluripotent Stem Cell Function of the Mouse Marrow "Lymphocyte"Science, 1964