Effect of Salicylates on Respiration and Phosphorylation in Heart Mitochondria

Abstract

The polarographic method for measuring changes in O2 concentration was employed for assessing the ability of salicylates and related compounds to uncouple phosphorylation. The extent of stimulation of respiration caused by addition of these compounds to rat heart mitochondria oxidizing [alpha]-ketoglutarate in a phosphate-acceptor deficient system was used as a criterion of uncoupling action. Thio-salicylate was many times more active than salicylate and other salicylate-derivatives tested. Some chemical and physical properties possibly responsible for the uncoupling action of salicylate and certain of its congeners were considered. The in vitro action of salicylates was discussed in relation to their antirheumatic activity and cardiovascular effects.