Polymarker, HLA-DQA1, and D1S80 Allele Frequency Data in Chamorro and Filipino Populations from Guam

Abstract

Allele frequencies were determined in sample populations of Chamorros and Filipinos from Guam at the loci LDLR, GYPA, HBGG, D7S8, Gc, HLA-DQA1, and D1S80. Variable number tandem repeat alleles at the D1S80 locus were detected by silver staining following electrophoresis of amplified products in polyacrylamide. Allelic products of the other loci were detected by reverse dot blot hybridization following a multiplex amplification procedure. All loci, in both sample populations, are highly polymorphic and meet Hardy-Weinberg expectations, except for the D1S80 locus in the Chamorro population sample (p = 0.025). An interclass correlation analysis detected only one marginally significant departure from independence out of a total of 42 pairwise comparisons of the seven loci for both data sets (LDLR/HBGG in Chamorros, p = 0.048). The Chamorro and Filipino allele frequency data are similar to each other at six of the seven loci with only a marginally significant difference at the HLA-DQA1 locus (p = 0.049).