American elm was propagated from callus derived from cell suspension cultures. Suspensions, established from callus, were plated onto agar where, after transfer to a simpler defined medium over 18 months, shoots were produced. Maintenance of high auxin levels yielded roots but no shoots. Shoots were removed from the callus, were treated briefly with indole-3-butyric acid, and transferred to a sphagnum moss – sand mixture for rooting. Other than the absence of cotyledons, plants from callus were comparable to elm seedlings.