Antigen-indued histamine release from peritoneal mast cells of mice producing reagin-like antibody.

Abstract

The time course of production in the mouse circulation, after different schemes of immunization, of reagin-like and γ1 antibodies, as measured by the ability of the antisera to induce thermolabile long-latence and thermostable short-latence PCA reactions, has been studied. γ1 antibody was obtained in all schemes of immunization and its amount in antiserum increased with time. Reagin-like antibody appeared on the 7th day after immunization with sufficient dose of ovalbumin and adjuvant, and on later days with less efficient antigens such as DNP—BSA and DNP—BGG. Maximal amounts of the reagin-like antibody were reached in the early days after immunization and its presence in the circulation had a somewhat transient character; however, it persisted in the circulation for periods of more than 60 days, on occasion.