Formation of 7-cis- and 13-cis-retinal pigments by irradiating squid rhodopsin
- 17 April 1979
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 18 (8), 1449-1453
- https://doi.org/10.1021/bi00575a010
Abstract
Squid [Todarodes pacificus] rhodopsin was irradiated with orange light (> 530 nm) at various temperatures from -190 to 10.degree. C until a photo steadystate mixture was formed. The chromophoric retinals were extracted from the photo steadystate mixtures and their isomer composition was analyzed by high-performance liquid chromatography. In the case of a photo steadystate mixture formed at -85.degree. C, large peaks in the chromatogram were found at the positions 7-cis- and 13-cis-retinals. Each peak was further identified by synthesizing the pigments from these retinals with cattle opsin or apobacteriorhodopsin. Both 7-cis- and 13-cis-retinals were extracted from a photo steadystate mixture formed by irradiation at -40, at 0 or at 10.degree. C. These isomers were scarcely detected in a photo steadystate mixture formed by irradiation at -190.degree. C, though 9-cis-retinal was a major constituent in this mixture. Irradiation of lumirhodopsin at -190.degree. C, produced 7-cis-retinal pigment. Bathorhodopsin may have a conformation to prevent the formation of 7-cis-retinal from the all-trans form and this particular conformation may be relaxed by the conversion of bathorhodopsin to lumirhodopsin.This publication has 9 references indexed in Scilit:
- SQUID HYPSORHODOPSINPhotochemistry and Photobiology, 1979
- Formation of 7-Cis Retinal by the Direct Irradiation of All-Trans Retinal1The Journal of Biochemistry, 1978
- Isorhodopsin: Conformation and orientation of its chromophore in frog disk membraneVision Research, 1978
- Isomeric Composition of Retinal Chromophore in Dark-Adapted Bacteriorhodopsin1The Journal of Biochemistry, 1977
- Sterically hindered isomers of retinal from direct irradiation of the all-trans isomer. Isolation of 7-cis-retinalJournal of the American Chemical Society, 1977
- A simple and non-isomerizing procedure for the identification of protein-linked retinalsExperimental Eye Research, 1977
- Studies on cephalopod rhodopsin: Photoisomerization of the chromophoreBiochimica et Biophysica Acta (BBA) - General Subjects, 1976
- Transformations of Squid Rhodopsin at Low TemperaturesNature, 1964
- CIS-TRANS ISOMERS OF VITAMIN A AND RETINENE IN THE RHODOPSIN SYSTEMThe Journal of general physiology, 1952