Self-assembly of synthetic phytochrome holoprotein in vitro
- 1 August 1989
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 86 (15), 5778-5780
- https://doi.org/10.1073/pnas.86.15.5778
Abstract
The phytochrome holoprotein of plants requires a covalently bound linear tetrapyrrole (bilin) prosthetic group for its photoreceptor function. Here we show that synthetic phytochrome apoprotein prepared by transcription and translation of an Avena phytochrome cDNA construct combines in vitro with phycocyanobilin, an analog of the natural chromophore, to produce a photoactive holoprotein. These results indicate that holprotein assembly is an "autocatalytic" process.This publication has 11 references indexed in Scilit:
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