Immortalization of Primary Human Epithelial Cells by Cloned Cervical Carcinoma DNA Containing Human Papillomavirus Type 16 E6/E7 Open Reading Frames
- 1 May 1989
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 70 (5), 1261-1266
- https://doi.org/10.1099/0022-1317-70-5-1261
Abstract
Summary Primary human epithelial cells were transfected with a subgenomic fragment of DNA cloned from a cervical carcinoma, containing the putative transforming genes E6 and E7 from the human papillomavirus type 16 prototype. Several immortalized cell lines were generated, all retaining tumour DNA and expressing the viral genes, suggesting that either one or both of these genes is sufficient for the immortalization of primary human genital keratinocytes.Keywords
This publication has 21 references indexed in Scilit:
- Identification of Human Papillomavirus Type 18 E6 Polypeptide in Cells Derived from Human Cervical CarcinomasJournal of General Virology, 1987
- The major human papillomavirus protein in cervical cancers is a cytoplasmic phosphoproteinJournal of Virology, 1987
- Identification of the HPV-16 E6 protein from transformed mouse cells and human cervical carcinoma cell lines.The EMBO Journal, 1987
- Transformation of human fibroblasts and keratinocytes with human papillomavirus type 16 DNAJournal of Virology, 1987
- Identification of early proteins of the human papilloma viruses type 16 (HPV 16) and type 18 (HPV 18) in cervical carcinoma cells.The EMBO Journal, 1987
- Transcription of human papillomavirus type 16 early genes in a cervical cancer and a cancer-derived cell line and identification of the E7 protein.Proceedings of the National Academy of Sciences, 1986
- Transforming activity of human papillomavirus type 16 DNA sequence in a cervical cancer.Proceedings of the National Academy of Sciences, 1986
- Human papillomavirus type 16 DNA-induced malignant transformation of NIH 3T3 cellsJournal of Virology, 1986
- Cellular Transformation by Human Papillomavirus DNA in VitroScience, 1984
- A new type of papillomavirus DNA, its presence in genital cancer biopsies and in cell lines derived from cervical cancer.The EMBO Journal, 1984