Bromsulphalein Fractional Clearance in Dairy Cattle as a Criterion of Liver Function, and the Simultaneous Determination of Volumes of Plasma and Blood

Abstract

The dye, bromsulphalein (BSP), was used in a procedure for the simultaneous determination of liver function, and volumes of plasma and blood in diary cattle. Bromsulphalein was eliminated from the blood of cattle in an exponential manner, the loge of dye concentration being related to the time after dye administration, in a rectilinear manner. The slope of the regression curve was termed the"BSP fractional clearance,"and was defined as the fraction of the total blood plasma volume which was cleared of BSP per minute on an instantaneous basis. Since the BSP distribution volume is the same as the plasma volume, blood and plasma volumes also may be determined using dye dilution techniques.