PROPAGATION OF INFECTIOUS BRONCHITIS VIRUS IN TISSUE CULTURE

Abstract
Two strains of infectious bronchitis virus were propagated serially in stationary flask cultures of chick embryo tissues. Both strains of virus could be grown in tube cultures of chick embryo tissues. Virus was produced by the cells for approximately one month without any evidence of damage to the cells. The strains tested could also be propagated in monkey kidney tissue cultures but not the L strain of cells. In monkey kidney cultures, virus was produced continuously for over one month without apparent damage to the cells.