ANTIMONY TRICHLORIDE – ETHANOL PRECIPITATION FOR THE FLUOROMETRIC DETERMINATION OF RIBOFLAVIN IN PORK

Abstract

A fluorometric method suitable for routine analyses is presented in which interfering substances in papain–takadiastase extracts of pork are precipitated in the presence of 0.02% antimony trichloride and 47.5% ethanol. Antimony trichloride prevents the adsorption of riboflavin upon the precipitate. Recoveries of 95 to 100% are obtained. Determinations by this method correlate well with those obtained microbiologically: for eight samples of fresh pork, four cooked, and four uncooked, r =.94; for 20 samples of cured pork, four cooked, and 16 uncooked, r =.98.