Rabies Virus Glycoprotein Analogs: Biosynthesis in Escherichia coli
- 11 February 1983
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 219 (4585), 614-620
- https://doi.org/10.1126/science.6297004
Abstract
The surface of rabies virus is composed of an approximately 60,000 dalton glycoprotein, in which most of the antigenic and immunogenic determinants of the virus reside. We have constructed plasmids for the direct expression in Escherichia coli of the mature full length rabies glycoprotein gene and also for the expression of a glycoprotein gene which has been truncated to exclude the coding region for a hydrophobic, possibly transmembrane, domain of the protein. Escherichia coli harboring the plasmids synthesize analog proteins which conform by several biochemical and antigenic criteria to rabies glycoprotein.Keywords
This publication has 26 references indexed in Scilit:
- Antibodies That React with Predetermined Sites on ProteinsScience, 1983
- Protection against foot-and-mouth disease by immunization with a chemically synthesized peptide predicted from the viral nucleotide sequenceNature, 1982
- Expression of human immune interferon cDNA in E. coli and monkey cellsNature, 1982
- Amino acid composition and terminal sequence analysis of the rabies virus glycoprotein: Identification of the reading frame on the cDNA sequenceBiochemical and Biophysical Research Communications, 1981
- Human leukocyte interferon produced by E. coli is biologically activeNature, 1980
- A Technique for Expressing Eukaryotic Genes in BacteriaScience, 1980
- The Structural Proteins of Rabies Virus and Evidence for their Synthesis from Separate Monocistronic RNA SpeciesJournal of General Virology, 1980
- Use of Hybridoma Monoclonal Antibodies in the Detection of Antigenic Differences Between Rabies and Rabies-related Virus Proteins. II. The GlycoproteinJournal of General Virology, 1980
- Synthesis in E. coli of a polypeptide with human leukocyte interferon activityNature, 1980
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979