Lack of Correlation between pp60src Kinase Activity and Transformation Parameters in Cells Infected with Temperature-conditional Mutants of Rous Sarcoma Virus

Abstract

The pp60src kinase activity of [chicken embryo] cells infected with temperature-conditional mutants of Rous sarcoma virus (RSV) which induce only a partial transformation was compared to the pattern of transformation parameters induced by these mutants. The tsGI251-infected cells were thermosensitive for hexose transport, fibrinolysis, morphological alterations and pp60src kinase activity, but cold-sensitive for loss of growth control. The tsGI253-infected cells were similar to tsGI251-infected cells except that they were only slightly cold-sensitive for loss of growth control and had a very low pp60src kinase activity which was temperature-insensitive. Thus the pp60src kinase activity measured in vitro with IgG as a substrate did not correlate in a consistent way with any of the measured parameters of transformation.