Ribosylzeatin and zeatin in tobacco crown gall tissue
- 1 January 1980
- journal article
- research article
- Published by Springer Nature in Planta
- Vol. 147 (4), 269-273
- https://doi.org/10.1007/bf00379831
Abstract
Cytokinins were extracted from two cultures of tobacco crown gall tumor tissue: an unorganized tissue and a teratoma which produced leafy shoots. On Sephadex LH-20 column chromatography, extracts of both types of tissue yielded two peaks of cytokinin activity with elution volumes similar to ribosylzeatin and zeatin. Ribosylzeatin and zeatin were detected and quantified by coupled gas chromatography — mass spectrometry selected ion monitoring (GC/MS SIM), comparable quantities being found in the two extracts. Full mass spectral evidence for the presence of ribosylzeatin in both tissues was obtained. No evidence was found for the presence of N6-(Δ2-isopentenyl)adenosine (i6Ade) or N6-(Δ2-isopentenyl)adenine (i6Ade) although these compounds have been reported to occur in cytokinin-habituated tobacco callus tissues.Keywords
This publication has 13 references indexed in Scilit:
- Opine content of unorganised and teratomatous tobacco crown gall tissuesPlant Science Letters, 1979
- Interactions and DNA transfer between Agrobacterium tumefaciens , the Ti-plasmid and the plant hostProceedings of the Royal Society of London. B. Biological Sciences, 1979
- Incorporation of 3H-adenine into free cytokinins by cytokinin-autonomous tobacco callus tissueBiochemical and Biophysical Research Communications, 1978
- The occurrence of raphanatin as an endogenous cytokinin in radish seed Identification and quantitation by gas chromatographic-mass spectrometric analysis using deuterium-labelled standardsFEBS Letters, 1977
- A specific radioimmunoassay for nanogram quantities of the auxin, indole-3-acetic acidPlanta, 1977
- Cytokinins in Vinca rosea L. Crown Gall Tumor Tissue as Influenced by Compounds Containing Reduced NitrogenPlant Physiology, 1976
- Nuclear DNA content and phytohormone requirements of normal, crown gall and habituated tissues of Nicotiana tabacum var. White BurleyExperimental Cell Research, 1974
- N6-(Δ2-Isopentenyl)adenosine: Its Occurrence as a Free Nucleoside in an Autonomous Strain of Tobacco TissuePlant Physiology, 1972
- Chromatographic Analysis of a Cytokinin from Tissue Cultures of Crown-GallPlant Physiology, 1971
- The caryological analysis of plants regenerated from tumorous and other callus cultures of tobaccoMolecular Genetics and Genomics, 1969